Functional Characterization of Alanine Racemase from Schizosaccharomyces pombe : a Eucaryotic Counterpart to Bacterial Alanine Racemase
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چکیده
منابع مشابه
Studies on spore germination: its independence from alanine racemase activity.
The demonstration in the past few years that simple nutrients are sufficient to enable a rapid germination of spores from aerobic bacteria has stimulated an interest in the biochemical reactions involved. Glucose, adenosine, and certain amino acids, added singly or in combination, were found by a number of workers to be sufficient to lead to over 90 per cent germination of spores from a number ...
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Among the archaea, Methanococcus maripaludis has the unusual ability to use L- or D-alanine as a nitrogen source. To understand how this occurs, we tested the roles of three adjacent genes encoding homologs of alanine dehydrogenase, alanine racemase, and alanine permease. To produce mutations in these genes, we devised a method for markerless mutagenesis that builds on previously established ge...
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The cyclic tetrapeptide HC-toxin is an essential virulence determinant for the plant pathogenic fungus Cochliobolus carbonum and an inhibitor of histone deacetylase. The major form of HC-toxin contains the D-isomers of Ala and Pro. The non-ribosomal peptide synthetase that synthesizes HC-toxin has only one epimerizing domain for conversion of L-Pro to D-Pro; the source of D-Ala has remained unk...
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Alanine racemase, an enzyme important to bacterial cell wall synthesis, is irreversibly inactivated by 3-chloro- and 3-fluorovinylglycine. Using alanine racemase purified to homogeneity from Escherichia coli B, the efficient inactivation produced a lethal event for every 2.2 +/- 0.2 nonlethal turnovers, compared to 1 in 800 for fluoroalanine. The mechanism of inhibition involves enzyme-catalyze...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2001
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.183.7.2226-2233.2001